Skills and Techniques:

This section gives an overview of some of the scientific techniques I have regularly performed at Imperial College London and The University of Medicine and Dentisty, New Jersey. The list is by no means comprehensive, but illustrates the laboratory techniques I am most familiar with. In addition, I have also listed some of my other skills I have been trained in, including teaching and lecturing.

 

Scientific Technical Skills

- Mammalian Cell Culture of many primary cells and cell lines

- Preparation and functional analysis of T cell subsets

Dendritic cells and Macrophages preparation and functional analysis

Flow Cytometry, analysis of Intracellular Cytokines and Tetramer studies

- Proliferation analysis using 3H-TdR and CFSE

- Cytokine analysis using ELISA, bioassay, ELISPOT and Protein Arrays

- Receptor mediated endocytosis and macropinocytosis assays

Immunohistochemistry and confocal laser scanning microscopy

Immunoprecipitation, Western Blotting and Silver Staining

Transduction, transfection and selection of transgenic cell lines

- Expression and purification of recombinant molecules

Development of ELISAs from hybridomas through to application

- Preparation of high grade nucleic acids for genetics studies

Real Time RT-PCR

Genotyping studies using Single Nucleotide Polymorphism (SNP) analysis

Bioinformatics of proteins and nucleotides

Bill Jordan

Science Skills and Techniques

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Bill Jordan

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